Cryopreservation of Ram Semen Facilitates Sperm DNA Damage: Relationship Between Sperm Andrological Parameters and the Sperm Chromatin Structure Assay
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چکیده
منابع مشابه
Relationship between reactive oxygen species production in human semen and sperm DNA damage assessed by Sperm Chromatin Structure Assay.
AIM The aim of this prospective study was to find possible relationship between ROS production measured by chemiluminescence and flow cytometry in human semen and sperm DNA damage estimated by Sperm Chromatin Structure Assay. METHODS Study included 39 men from infertile couples and 23 fertile volunteers who served as a control group. Aliquot of neat semen was used for ROS detection by chemilu...
متن کاملIncreased DNA damage in sperm from leukocytospermic semen samples as determined by the sperm chromatin structure assay.
OBJECTIVE To determine DNA damage as measured by the sperm chromatin structure assay (SCSA) in subsets of human spermatozoa at different stages of maturation in patients who are undergoing infertility evaluation. DESIGN Prospective study. SETTING Andrology laboratory at a tertiary care hospital. PATIENT(S) Fifty-six patients undergoing infertility evaluation. Patients with normal semen pa...
متن کاملSperm DNA damage and semen quality
Aim: To investigate the prevalence of high levels of sperm DNA damage among men from infertile couples with both normal and abnormal standard semen parameters. Methods: A total of 350 men from infertile couples were assessed. Standard semen analysis and sperm chromatin structure assay (SCSA) were carried out. Results: Ninety-seven men (28% of the whole study group) had a DNA fragmentation index...
متن کاملSperm DNA damage and semen quality impairment after treatment with selective serotonin reuptake inhibitors detected using semen analysis and sperm chromatin structure assay.
PURPOSE Semen parameters and sperm DNA were evaluated in patients receiving selective serotonin reuptake inhibitors. MATERIALS AND METHODS Semen samples were obtained from 74 fertile, depressed men treated with selective serotonin reuptake inhibitors (group 1) and 44 healthy fertile volunteers who served as a control (group 2). Two semen analyses and physical examinations were completed in al...
متن کاملSperm Chromatin Structure Assay (SCSA®): The Clinical Utility of Measuring Sperm DNA Damage and its Potential Improvement with Supplemental Antioxidants
The Sperm Chromatin Structure Assay (SCSA®) is the pioneering sperm DNA fragmentation assay that precisely measures the percent of sperm in a semen sample that have Sperm DNA Damage (SDD), a negative factor for successful pregnancy. The SCSA® is a rapid, dual parameter, computer driven assay with diligent unbiased flow cytometer measurements on five to ten thousand sperm per sample providing ri...
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ژورنال
عنوان ژورنال: Journal of Andrology
سال: 2004
ISSN: 0196-3635
DOI: 10.1002/j.1939-4640.2004.tb02782.x